WP2: B-cell assays in health and disease
WP2 is dedicated to set-up specialized cellular assays to study the dynamics, diversity and function of B-lymphocyte differentiation in health,
B-IMDs and B-ONC. We are building a powerful, state-of-the-art, B-cell immune monitoring platform based on integration of existing and novel assays for immune monitoring of B-cell function and differentiation in health and B-IMDs/B-ONCs. These assays include deep-immunophenotyping of B cells at a single cell level, using CyTOF and scRNA-sequencing, and functional analyses by investigating differentiation and signaling of B cells.
With this Target-to-B!-dedicated platform, we can characterize the B-cell compartment in healthy individuals and B-IMD/B-ONC patients, to assess
B-cell function, pathogenicity and/or recovery in our clinical cohorts (WP1) and subsequent in-depth analysis in dedicated lines of research (WP5-8). The strong collaborations and interactions with the other work packages stimulate to efficiently build this immune monitoring platform. It will allow us to investigate the contribution of the B-cell/plasmablast and plasma cell compartment in many B-IMD and B-ONC and compare results, thus yielding a truly disease-overarching B-cell immunomonitoring platform.